Effects of Combination of SAHA and TRAIL on Proliferation of Triple-negative Breast Cancer Cell MDA-MB-231

VernacularTitle:SAHA和TRAIL联合使用对三阴性乳腺癌细胞MDA-MB-231生长状态的影响
Author: Han HAN ; Jia XU
Publication Type:Journal Article
Keywords: suberoylanilide hydroxamic acid; TNF-related apoptosis inducing ligand; triple-negative breast cancer cell
From: Journal of China Medical University 2016;45(7):591-595,600
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of combined treatment of suberoylanilide hydroxamic acid(SAHA)and TNF?related apoptosis inducing ligand(TRAIL)on proliferation and morphology change of triple?negative breast cancer cell MDA?MB?231. Methods The effects of combination treatment of SAHA and TRAIL on proliferation and morphology change of MDA?MB?231 cells were monitored by RTCA. Morphology changes of MDA?MB?231 cells by different treatment factors were observed through time?lapse live cell imaging acquisition. Results Real?time cell proliferation assays showed that a synergistic effect were found when MDA?MB?231 cells were treated with combination of SAHA and TRAIL , and reached the best effect with 5μmol/L SAHA and 50 ng/mL TRAIL. The results of time?lapse live cell imaging acquisition showed that the growth inhibition of MDA?MB?231 cells with combined treatment of SAHA and TRAIL were more obvious than that with treatment of SAHA or TRAIL alone. Conclusion The combined treatment of SAHA and TRAIL induces a synergistic effect on growth inhibition in triple?negative breast cancer cell line MDA?MB?231.