Effects of heat shock protein 70 on mice with traumatic brain injury-related acute gastric mucosal lesions
10.7652/jdyxb201604015
- VernacularTitle:热休克蛋白70对小鼠颅脑创伤相关急性胃黏膜病变的保护作用
- Author:
Guizhong YAN
;
Dengfeng WANG
;
Wenzhen YANG
;
Jixing LIU
;
Boru HOU
;
Haijun REN
- Publication Type:Journal Article
- Keywords:
traumatic brain injury (TBI);
acute gastric mucosal lesions;
heat shock protein 70;
apoptosis
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2016;37(4):547-550,589
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective mechanism of HSP70 protein in traumatic brain injury (TBI)‐related acute gastric mucosal lesions in mice .Methods Forty adult male Balb/c mice were randomly divided into sham (A) ,TBI (B) ,TBI+ geranylgeranylacetone (GGA) (C) ,and TBI+saline (D) groups .TBI was induced via the Feeney impact model .GGA (800 mg/kg) was administered via oral tube beginning before the model was built in group C .The expressions of HSP70 protein in brain and gastric mucosa were determined by immunohistochemistry , and the apoptotic index was detected by TUNEL method .Results The injury area in mouse brain and gastric mucosa was greater in group B than in groups A and C (P<0 .05) .After model induction ,the content of HSP70 protein in group B was markedly higher in the brain and gastric mucosa ,which was notably higher than in group A (P<0 .05) .Obviously apoptotic cells were observed in groups B and D ,which were significantly higher than in groups A and C .GGA pretreatment enhanced the up‐regulated expression of HSP70 and decreased the apoptotic index distinctly ;HSP70 expression was higher in group C than in groups B and D ,but the apoptotic index was lower (P<0 .05) .Conclusion GGA can induce HSP70 protein expression in mouse brain and gastric mucosa .HSP70 is involved in the process of apoptosis inhibition .GGA can be used in the prevention and therapy of TBI‐related acute gastic mucosal lesions .
