Influence of Bufalin on the Growth and Proliferation of Human Glioma Cell Line U251
- VernacularTitle:蟾毒灵对人脑胶质瘤 U251细胞生长增殖的影响
- Author:
Yanhua WANG
;
Yan WENG
;
Jianjie CHU
;
Miaomiao XI
;
Zhifu YANG
;
Aidong WEN
- Publication Type:Journal Article
- Keywords:
Bufalin;
Human glioma cell;
Proliferation inhibition;
Cell apoptosis
- From:
China Pharmacist
2016;19(5):825-827,828
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of Bufalin on the growth and proliferation of human glioma cells U251. Methods:Methyl thiazolyl tetrazolium(MTT)assay was used to detect the effect of Bufalin on the proliferation of human glioma cells U251. An in-verted microscope was used to observe the changes of cell number,morphology and activity. AnnexinV/ PI was used to measure the in-duction of cell apoptosis caused by Bufalin. Results:Bufalin at different concentrations(0. 001 - 10. 0μmol·L - 1 )inhibited the pro-liferation of U251 cells in a dose and time-dependent manner. Compared with that of the control group,the apoptosis rate of Bufalin group was increased significantly(P < 0. 01). Conclusion:Bufalin can inhibit the growth and proliferation of U251 cells in a dose and time-dependent manner,and induce the apoptosis of U251 cells.
