In vitro Corneal Permeation and Antibacterial Activity of N-Trimethyl Chitosan-coated Sparfloxacin Lac-tate Nanoliposomes
- VernacularTitle:N-三甲基壳聚糖包覆乳酸司帕沙星纳米脂质体体外角膜渗透性及抗菌性
- Author:
Ling SONG
;
Wen HE
- Publication Type:Journal Article
- Keywords:
Sparfloxacin lactate;
Nanoliposomes;
N-Trimethyl chitosan;
Corneal permeation;
Antibacterial activity
- From:
China Pharmacist
2016;19(5):863-865,883
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the in vitro corneal permeation and antibacterial activity of N-trimethyl chitosan(TMC)-coated sparfloxacin lactate(SL)nanoliposomes. Methods:Franz diffusion cells were used with rabbit cornea as the barrier to study the in vitro corneal permeation of TMC-coated SL nanoliposomes,and the permeation parameters were calculated. Eseheriehia eoli,staphylo-eoeeus aureus,pseudomonas aeruginosa and baeillus subtilis were used as the tested bacterial strains and the in vitro antibacterial activity of the SL preparations was investigated to obtain the minimum inhibitory concentration(MIC),the minimum bactericidal concentration (MBC)and the relationship of bacterial inhibitory rate and time. Results:The order of steady permeation rate(J),corneal perme-ation coefficient(P)and corneal retention rate was TMC-coated SL nanoliposomes > SL nanoliposomes > SL eye drop. The order of lag time(τ)and corneal diffusion coefficient(D)was SL nanoliposomes > SL eye drop > TMC-coated SL nanoliposomes. The in vitro an-tibacterial activity showed the order of TMC-coated SL nanoliposomes > SL nanoliposomes > SL eye drop. Conclusion:Compared with the eye drop,liposomes can enhance the corneal permeation and storage of SL with improved antibacterial activity,and TMC-coating can further improve the permeation and antibacterial activity,which is worthy of further study.
