Effect of Sanhuang Decoction on glucose and lipid metabolism in 3 T3-L1 adipocytes
10.3969/j.issn.1001-1978.2016.06.025
- VernacularTitle:三黄汤对3 T3-L1脂肪细胞糖脂代谢的影响
- Author:
Qingfeng LIU
;
Yi LIU
;
Zheng JIAO
;
Zhongdong LI
;
Xiaojin SHI
;
Mingkang ZHONG
- Publication Type:Journal Article
- Keywords:
Sanhuang Decoction;
glucose;
triglycer-ide;
glycerol;
free fatty acid;
GLUT-4;
3T3-L1 adipo-cytes
- From:
Chinese Pharmacological Bulletin
2016;32(6):868-872,873
- CountryChina
- Language:Chinese
-
Abstract:
Aim ToobservetheeffectofSanHuang Decoction (SHD )on glucose and lipid metabolism in insulin resistance(IR)3T3-L1 adipocytes.Methods TheIRmodelof3T3-L1adipocyteswasinducedby high glucose and hyperinsulinism cultivation(also con-taining dexamethasone ).The adipocytes were treated with rosiglitazone(Ros)and different concentrations of SHD(2. 5,5,10,20,40 g·L-1 )for 24 h.The content of glucose disappeared from the culture medium was determined as glucose consumption of the cells. The transport of glucose was observed by 2-deoxidation-[3 H]-glucose uptake method.The efflux of nonesteri-fied fatty acids(NEFA)from adipocytes was observed by the concentration of NEFA in the culture medium. The mRNA expression of glucose transporter-4 (GLUT-4)was measured by real-time polymerase chain reac-tion (real-time PCR).The protein expression of GLUT-4wasdetectedbyWesternblot.Results Compared with the Con group,SHD(5,10,20,40 g·L-1 ) could significantly induce the glucose consumption and transportion(P<0. 01 ),increase the glycerol content in culture supernatant(P<0. 01 ),reduce the NEFA concentration in culture medium and accumulation of intracellular TG(P<0. 01 ).And all of these change showed a concentration-dependent effect when SFD concentrations were from 5 g·L-1 to 20 g·L-1 .At the same time,Ros could also remarkably induce the glucose consumption and transportion(P<0. 01 ),and increase the accumulation of intracellular TG (P <0. 01);but the concentration of NEFA and glycerol in culture medium did not show any difference in the Ros-treatedgroup(P>0.05).Conclusion SHDcanin-crease insulin sensitivity by increasing glucose trans-portation and consumption in the 3T3-L1 adipocytes as well as decreasing the NEFA efflux from the cells.
