The effect of LPS on VEGF expression in microglia
10.11958/20150374
- VernacularTitle:脂多糖对小胶质细胞血管内皮生长因子表达的影响
- Author:
Yong ZENG
;
Jingfang LYU
;
Gongjie YU
;
Weiwei GAO
;
Jianning ZHANG
- Publication Type:Journal Article
- Keywords:
lipopolysaccharides;
microglia;
vascular endothelial growth factors;
hypoxia-inducible factor 1,alpha sub-unit
- From:
Tianjin Medical Journal
2016;44(6):669-671,648
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether lipopolysaccharide (LPS) can induce vascular endothelial cell growth factor (VEGF) expression in microglia regulated by hypoxia inducible factor-1α(HIF-1α). Methods The cultured BV2 cells were divided into four groups:control group, LPS (100 μg/L) simulated group, LPS (100 μg/L)+LPS antagonist (LRS, 200 μg/L) intervened group and LPS (100 μg/L)+HIF-1αinhibitors FM19G11 (10 mmol/L) intervened group. Immunofluorescence staining, Western blotting and ELISA were used to detect the expressions of VEGF and HIF-1α. Results Compared with the control group, the VEGF expression level was obvious high in LPS simulated group (P<0.05). LRS inhibited this effect of LPS (P<0.05). The HIF-1αlevel was increased in LPS simulated group at 8 h post-injury (P<0.05). FM19G11, the inhibitor of HIF-1αreduced the expression of VEGF induced by LPS (P<0.05). Conclusion LPS can up-regulate the expression of VEGF by HIF-1α.
