Expression and Significance of Mcl-1 Gene in Hepatocellular Carcinoma
10.3969/j.issn.1671-7414.2016.03.019
- VernacularTitle:髓样细胞白血病-1基因在原发性肝癌组织中的表达及临床意义
- Author:
Feng LI
;
Qun WEI
;
Guoxiong ZHOU
;
Dongfeng HUANG
;
Hong ZHANG
- Publication Type:Journal Article
- Keywords:
myeloid cell leukemia-1 gene;
primary hepatocellular carcinoma;
liver cirrhosis;
RT-PCR;
Western blot;
immuno-histochemistry
- From:
Journal of Modern Laboratory Medicine
2016;31(3):69-72,76
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significance of myeloid cell leukemia-1 (Mcl-1 )gene and protein in Hepatocellular Carcinoma(HCC).Methods The expression of Mcl-1 was detected respectively by Reverse Transcription-Polymerase Chain Reaction (RT-PCR),Western blot and ENVISION immunohistochemistry in 20 HCC specimens,19 liver cirrhosis(LC)specimens,and 12 control ones.Their relationship with clinical and pathological characteristics of HCC was investigated.Results The expression of Mcl-1 mRNA in the control group,LC group and HCC group was 0.52±0.17, 3.46±1.7,3.65±2.93,respectively.The level in HCC and LC group was statistically different compared with the control group,respectively (t=7.925,5.334,P<0.05).The relative expression of Mcl-1 protein in LC group (0.51±0.35)and HCC group (0.75±0.36)were significantly higher than that in the control group (0.21±0.19)(t=5.526,6.355,P<0.05).The positive expression rate of Mcl-1 in HCC group was 55.00% (11/20),significantly higher than that in the con-trol group 33.33% (4/12)(t=7.835,P<0.05).The positive expression of Mcl-1 was related to tumor necrosis and TNM staging (χ2=4.201,P<0.05).Conclusion Mcl-1 gene and protein are differentially expressed in HCC,LC and the control, which may be involved in the occurrence,development and malignant transformation of HCC.
