Effect of excretory/secretory protein of Trichinella spiralis adult worm on CLP-induced sepsis in mice
10.16250/j.32.1374.2016080
- VernacularTitle:旋毛虫成虫排泄分泌蛋白抗CLP诱导的小鼠脓毒症的观察
- Author:
Xiaodi YANG
;
Wenxin HE
;
Qiang FANG
;
Di SONG
;
Qi WU
;
Xiaoli WANG
;
Nan LI
;
Qi QI
;
Yongkun WAN
;
Hui ZHANG
;
Rui ZHOU
;
Xingzhi CHEN
;
Mulin LIU
;
Huihui LI
;
Liang CHU
- Publication Type:Journal Article
- Keywords:
Trichinella spiralis;
Cecal ligation and puncture(CLP)operation;
Excretory/secretory products;
Sepsis;
Pro-inflammatory cytokine;
Anti-inflammatory cytokine
- From:
Chinese Journal of Schistosomiasis Control
2016;28(3):293-296,322
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of excretory/secretory products from Trichinella spiralis adult worms(AES)on cecal ligation and puncture(CLP)?induced sepsis in mice. Methods Forty?eight BALB/c mice were randomly divided into 3 groups:a sham operation group(PBS+sham group,Group A),a CLP?induced sepsis group(PBS+CLP group,Group B)and an AES treatment group(AES+ CLP group,Group C). The mice of each group were intraperitoneally injected with 25 μg of AES or PBS only as a control in a total volume of 200μl. Eight mice from each group were selected randomly for survival analy?sis of 96 hours. The other 8 mice in each group were observed for pathological changes in the lung,liver and kidney tissues by HE staining 12 h after CLP,and then determined for the detection of cytokines including TNF?α,IL?1β,IL?6,IL?10 and TGF? βin the sera by ELISA. Results The difference among the survival rates of mice in the 3 groups was statistically significant (χ2=21.16,P<0.05). Compared to Group A(100%),the survival rate of mice in Group B(0)decreased significantly(P<0.05),and also the pathological damage degrees in the lung,liver and kidney tissues of the mice in Group B increased signifi?cantly after CLP. Compared with the mice in group B,the survival rate of those in Group C(70%)increased significantly(P<0.05),and the pathological damage degrees in the lung,liver and kidney tissues of the mice in Group C decreased significantly after the treatment with AES. The differences among the levels of pro?inflammatory cytokines TNF?α(F=27.11,P<0.05),IL?1β(F=18.75,P<0.05)and IL?6(F=100.93,P<0.05)in the sera of the mice in the three groups were statistically signifi?cant. Compared with the mice in Group A,the levels of the 3 cytokines of those in Group B increased significantly(all P <0.05). However,after the treatment with AES,the levels of the pro?inflammatory cytokines of those in Group C decreased signifi?cantly(all P<0.05). The differences among the levels of immunoregulatory cytokines IL?10(F=10.88,P<0.05)and TGF?β(F=11.37,P<0.05)in the sera of the mice in the three groups were also statistically significant. Compared with the mice in Group B,the levels of IL?10 and TGF?β of those in Group C were higher after treatment with AES(both P<0.05). Conclu?sion T. spiralis AES has a therapeutic potential for alleviating sepsis induced by CLP in mice.
