Preparation and Identification of A Peptide Monoclonal Antibody Against Human Papillomavirus 1 8E6
10.3969/j.issn.1671-7414.2016.03.009
- VernacularTitle:抗 HPV18 E6多肽单克隆抗体的制备及鉴定
- Author:
Qianqian KONG
;
Zhenhua TANG
;
Fenfen XIANG
;
Yueping ZHAN
;
Jian XU
;
Rong WU
;
Xiangdong KANG
- Publication Type:Journal Article
- Keywords:
human papillomavirus;
18E6;
polypeptide;
monoclonal antibody
- From:
Journal of Modern Laboratory Medicine
2016;31(3):30-32,35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a peptide monoclonal antibody (McAb)against human papillomavirus 18E6 separately,and identify its specificity and pathogenicity.Metheds The advantage epitope peptide was designed and synthesized by ABCpred and Bcepred,and then used to immunize BALB/c mice after coupling with bovine serum albumin (BSA).And the McAb was prepared by hybridoma technique.HPV18E6 gene was amplified from cervical swab specimen containing HPV18 and insert-ed into expression vector pET-28a.The constructed recombinant plasmid was transformed to E.coli BL21(DE3)for expres-sion under induction of isopropyl thio-β-D-galactoside.The expressed protein was used to identified the McAb had been pre-pared.Results The hybridoma cell lines could constantly produce MAbs against HPV18E6 peptides.Sequencing proved that recombinant plasmid pET-28a-HPV18E6 was constructed correctly.Western blotting showed that the anti-HPV18E6 pep-tides antibody could specifically recognize HPV18E6.Conclusion A monoclonal antibody against the advantage epitope pep-tide of human papillomavirus 18E6 prepared could specifically recognize HPV18E6 specifically.
