Investigation on the Consistency of Five Measurement Systems for Serum Lipids
10.3969/j.issn.1671-7414.2016.03.011
- VernacularTitle:五种血脂检测系统测定结果的可比性调查
- Author:
Qian DI
;
Ling QIU
;
Lian HOU
;
Jun DONG
;
Fang ZHAO
;
Pengchang LI
;
Kai WANG
;
Xinqi CHENG
;
Xiuzhi GUO
- Publication Type:Journal Article
- Keywords:
total cholesterol;
triglyceride;
reference method
- From:
Journal of Modern Laboratory Medicine
2016;31(3):36-42,47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore achieving the consistent method of blood lipid examination by comparing the results of 5 dif-ferent blood lipid detection system commonly used in the use of refernce method to assign freach blood serum before and af-ter calibration.Methods Used the indoor quality control total variation (CV%)to evaluate the 5 blood lipid examination system of the imprecision.Referenced the United States Clinical and Laboratory Standardization Institution (CLSI)9A2 EP program,compared with 54 fresh blood serum in 5 commonly used examination system of Total Cholesterol (TC)and Tri-glyceride (TG),and then estimated the bias between the different detection systems and mean value.8 of the samples were determined by the reference method and estimate the bias of different system.The fresh frozen serum samples assigned by reference method were used to evaluate the above examination system,then compare and estimate the bias again with the same 54 fresh serum samples.Compared the variation of 54 samples in different detection system before and after calibra-tion.Results The TG imprecision of 5 examination system were between 3.76%~23.65%,the TC imprecision between 2.19%~23.43%,that mean the results were good,the r value of TG were between 0.996 7~0.999 6 and the TC were 0.956 2~0.996 7.But there were obvious differences between the results of the systems,and the biggest difference were 14.72%~34.21% in TG and 3.11%~14.57% in TC.After use the serum assignment by reference method,the variation of the systems has been significantly decreased.Conclusion Using the reference method to assign the fresh serum of different blood lipid detection system can effectively improve the consistency of the results.
