Devolepment of Real-time Fluorescence Quantitative RT-PCR Detection for Avian Influenza Virus H7N9 Type
10.3969/j.issn.1671-7414.2016.03.013
- VernacularTitle:禽流感病毒 H7N9型实时荧光定量RT-PCR检测方法的研究
- Author:
Mianqiao CHEN
;
Haoyang YU
- Publication Type:Journal Article
- Keywords:
avian influenza virus H7N9;
real-time fluorescence quantitative;
RT-PCR;
detection
- From:
Journal of Modern Laboratory Medicine
2016;31(3):48-50,54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid and accurate method of fluorescent quantitative PCR detection for Avian Influenza Virus H7N9 type.Methods A pair of primers and TaqMan fluorescent probes were designed specificly for H7N9 gene ac-cording to the published nucleotide sequence from National Center for Biotechnology Information.Positive recombinant plas-mid was built and the minimum copies of detection,repeatability and specificitv were tested.Results The results showed that this assay obtained positive recombinant plasmid,the linear relation was fine in the range from 102 to 108 copies/μl.The minimum copies was 500 copies/μl with this method,specificitv and repeatability(CV<1%)were fine,no cross reaction with other viruses.The precision was 100% (16/16)with this method for detecting 8 positive samples and 8 negative sam-ples.Conclution The establishment methods of fluorescent quantitative PCR detection could be used for the rapid diagnosis Avian Influenza virus H7N9 types.
