Expression of miR137 and its target gene Kruppel-like transcription factor 12 in multiple myeloma and their prognostic value

VernacularTitle:miR137及其靶基因Kruppel样转录因子12在多发性骨髓瘤中的表达及预后意义
Author: Shuaishuai ZHANG ; Yan XU ; Shuhui DENG ; Yu QIN ; Chenxing DU ; Xuehan MAO ; Lugui QIU
Publication Type:Journal Article
Keywords: Multiple myeloma; miR137; Kruppel-like factor 12
From: Journal of Leukemia & Lymphoma 2016;25(6):326-329,335
CountryChina
Language:Chinese
Abstract: Objective To explore the prognostic impact of miR137 target gene Kruppel-like transcription factor 12 (KLF12) in multiple myeloma (MM). Methods The target genes of miR137 were predicted by software. The GFP analysis of KLF12 and the prognosis of MM were constructed. Overexpressing miR137 in MM NCI-H929 cell line was also constructed. Real-time qPCR and Western blot were used to detect the expression of KLF12 in this cell line. Results The target genes of miR137 were MITF, BUE2H, SH3BP5 and KLF12. High expression of KLF12 in 455 patients included 75 patients (16.5 %) died, 104 patients with low expression of KLF12, and 25 patients (24.0 %) died, but no significance was detected in the different subgroups. KLF12 expression was higher in MM NCI-H929 cell line with miR137 over expression. The expression of miR137 was positively correlated with the expression of KLF12. Conclusion miR137-KLF12 is an important index to judge the prognosis of MM.