Interaction between bovine serum albumin and pullulan acetate nanoparticles
10.3760/cma.j.issn.1673-4181.2016.02.004
- VernacularTitle:乙酰普鲁兰纳米粒子与牛血清白蛋白的相互作用
- Author:
Hongbo TANG
;
Tong ZHANG
;
Xiaojun TAO
;
Bo DU
;
Xin FENG
;
Qiqing ZHANG
- Publication Type:Journal Article
- Keywords:
Pullulan acetate;
Bovine serum albumin;
Nanoparticles;
Fluorescence spectrum;
Circular dichroism
- From:
International Journal of Biomedical Engineering
2016;39(2):83-86,106,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the interaction of pullulan acetate nanoparticles (PANs) and bovine serum albumin (BSA),and to provide the basis for in vivo pharmacokinetic study of PANs.Methods Mixed solutions of different concentration of PANs and BSA solution was prepared,the interaction was studied by fluorescence quenching method and circular dichroism (CD) measurement.Apparent quenching constant (Kq) between the PANs and BSA was calculated,and the anti-stress effect induced by urea and heating of PANs-BSA solution was observed.Results The fluorescence spectrum of BSA was affected by PANs on density-related manner,and Kq calculated by the modified Stern-Volmer plot increased from 2.64×104 to 3.55 ×l05 with the concentration of PANs increased from 0.015 mg/ml to 0.25 mg/ml.With the denaturation of urea or heating,the CD spectrum of PANs-BSA and free BSA had the similar variation tendency.Conclusions The fluorescence spectrum display results show that interaction between PANs and BSA exists,but the interaction does not protect the BSA from degeneration by urea and heating.
