Effects of functionalization of multi-walled carbon nanotubes on human peripheral blood mononuclear cell
10.3760/cma.j.issn.1673-4181.2016.02.003
- VernacularTitle:功能化修饰多壁碳纳米管对人外周血单个核细胞的毒性研究
- Author:
Jingjing MA
;
Xiaoxuan LIU
;
Zhiting SUN
;
Lanxia LIU
;
Xigang LENG
- Publication Type:Journal Article
- Keywords:
Multi-walled carbon nanotubes;
Human peripheral blood mononuclear cell;
Cytotoxicity;
Functionalization
- From:
International Journal of Biomedical Engineering
2016;39(2):79-82,91
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of surface functionalized multi-walled carbon nanotubes (FMWCNTs) on the cytotoxicity of human peripheral blood mononuclear cell (PBMC).Methods Five different types of MWCNTs (hydroxylated,carboxylated,aminated,nickel-plated and pristine MWCNTs (P-MWCNTs)) with the same diameter and length were evaluated the dispersion and characterizations in physiological salt solution by transmission electron microscopy.PBMC were isolated by density gradient centrifugation from human peripheral blood,and 5 types of MWCNTs were ultrasonically dispersed in serum-containing medium respectively.After incubation with PBMC for 12,24,48 or 72 h,cytotoxicity was detected by CCK-8 kits.Results All the MWCNTs had well dispersion,especially the F-MWCNTs.Cytotoxicity results showed that all types of MWCNTs could induced PBMC death,and presented dose-dependence manner and a certain degree of time-dependence manner.Compared with the P-MWCNTs,F-MWCNTs changed cytotoxicity statistically,with the hydroxylated,carboxylated,aminated MWCNTs weakened,aminated MWCNTs significant (P＜0.05),nevertheless the nickel-plated MWCNTs increased.Compared with the P-MWCNTs (25 μg/ml),cell viability of PBMC after 24 and 48 h incubation with the same dose of nickelplated MWCNTs both decreased,and the differences was statistically significant (P＜0.01,P＜0.05).Conclusions The functional group modification affects not only the MWCNTs dispersion in medium,but also the cytotoxicity of the MWCNTs on PBMC.
