Protective effect of Ang-(1-7) on the permeability of blood brain barrier after subarachnoid hemorrhage
10.3969/j.issn.1671-8348.2016.17.007
- VernacularTitle:血管紧张素1-7对蛛网膜下腔出血后血脑屏障通透性的保护作用
- Author:
Jianmin ZHANG
;
Peng CHEN
;
Fanxi ZHANG
;
Xiaolong HUANG
;
Yufeng ZHOU
- Publication Type:Journal Article
- Keywords:
subarachnoid hemorrhage;
blood-brain barrier permeability;
Ang-(1-7);
vascular endothelial cell
- From:
Chongqing Medicine
2016;45(17):2327-2329,2333
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analysis the effects of Ang-(1-7) on the blood brain barrier permeability after subarachnoid hemor-rhage .Methods SAH-rats were produced by two times injection of blood into cisterna magna .Evans blue was used to detect the the permeability of SAH-rats brains and brain water content .RT-PCR and Western blot were performed to measure the expression of adhesion protein ICAM-1 and VCAM-1 in brains of SAH-rats .The artificial hemorrhagic cerebrospinal fluid (BCSF) was used to stimulate vascular endothelial cells (HBMEC) ,and the proliferation and apoptosis of HBMEC cell were analyzed .Results Ang-(1-7) reduced the content of Evans blue and brain water in brains of SAH-rats in dose and time dependent manner with the most sig-nificant change under the treatment of 10 - 5 mol/L Ang-(1-7) for 24 h (P< 0 .05) .Under the above condition ,the mRNA and pro-tein levels of ICAM-1 and VCAM-1 in brains of SAH-rats were significantly up-regulated (P< 0 .05) ,while the content of Evans blue in HBMEC cells stimulated by BCSF was obviously reduced .Besides ,Ang-(1-7) was observed to increase the expression of ICAM-1 and VCAM-1 in BCSF-stimulated HBMEC cells ,enhance the proliferation of HBMEC cells but reduce their apoptosis . Conclusion Ang-(1-7) plays a protective role in the blood-brain barrier damage induced by subarachnoid hemorrhage .
