Relationship between serum PAPP-A expression and gene polymorphism with severity of coronary lesions in patients with coronary heart disease
10.3969/j.issn.1671-8348.2016.18.012
- VernacularTitle:PA PP-A 的表达及基因多态性与冠心病患者冠状动脉病变程度的关系
- Author:
Hui LIU
;
Haijian LI
;
Chuanyu GAO
;
Shaofen MAO
;
Yafei TAO
;
Dongsheng REN
- Publication Type:Journal Article
- Keywords:
coronary heart disease;
pregnancy-associated plasma protein-A;
gene polymorphism;
severity of coronary artery lesion
- From:
Chongqing Medicine
2016;45(18):2488-2490,2493
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between serum pregnancy associated plasma protein A (PAAA‐A) ex‐pression and gene polymorphism with the severity of coronary lesions in the patients with coronary heart disease (CHD) .Methods Ninety‐eight patients with CHD in the Nanyang Municipal Central Hospital were selected as the observation group and divided into single vessel lesion group and multiple vessel lesion group according to coronary angiographic results .Ninety‐eight individuals un‐dergoing healthy physical examination were selected as the control group .The venous blood was collected at the visiting hospital in the observation group and at the physical examination in the control group for detecting the serum PAPP‐A protein level by ELISA .PAPP‐A gene and IVS6+ 95 polymorphism were analyzed by adopting polymerase chain reaction restriction fragment length polymorphism(PCR‐RELP) .Results Compared with the control group ,peripheral blood PAPP‐A protein level in the obser‐vation group was significantly increased(P<0 .05) ,moreover the PAPP‐A protein level in the multiple vessel lesion group was sig‐nificantly higher than that of the single vessel lesion group (P<0 .05) .The peripheral blood PAPP‐A level was positively correlated with the severity of CHD .Three genotypes existed in PAPP‐A gene IVS6+95 locus ,including CG heterozygous ,homozygous CC and GG homozygous type .The CC homozygous allele frequency in the patients with multiple vessel lesion was higher than that in the patients with single vessel lesion (P<0 .05) .Conclusion The PAPP‐A protein level and IVS6+95 polymorphism have a close relation with the severity of coronary lesions in patients with CHD .CC genotype may be one of genetic susceptibility gene markers in the patients with CHD .
