Research of the anti -inflammatory effects of sinomenine combined with methotrexate on adjuvant arthritis
10.3760/cma.j.issn.1008-6706.2016.14.005
- VernacularTitle:青藤碱联合甲氨蝶呤治疗佐剂性关节炎的研究
- Author:
Guiling XI
- Publication Type:Journal Article
- Keywords:
Sinomenine;
Methotrexate;
Adjuvant arthritis;
TNF -α;
IL -1β
- From:
Chinese Journal of Primary Medicine and Pharmacy
2016;23(14):2095-2097,2098
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti -inflammatory effects of combination of sinomenine and meth-otrexate on adjuvant arthritis (AA)in rats.Methods Rats were randomly divided into the model group,the sinomen-ine group(SIN):intragastric administration of the sinomenine,the methotrexate group(MTX),methotrexate combined with sinomenine(SIN +MTX).Another 12 rats were assigned as blank control group,gavage volume of saline.The arthritis index and the paws swelling were recorded,and the levels of IL -1β,TNF -αin serum were determined by ELISA.Results (1)Compared with the normal group,the arthritis index and the paws swelling were increased signif-icantly in the model group(all P <0.05).The serum levels of IL -1βand TNF -αwere also increased significantly in the model group(all P <0.05).(2)The combination treatment provided a dramatically inhibitory effect on second-ary reaction of AA,and the serum levels of IL -1βand TNF -αwere significantly decreased;(3)The combined med-icine group could not only control inflammation,but also effectively protect the bones by reducing the generating of osteoclasts.Conclusion The combined medicine group of sinomenine and methotrexate can suppress AA rats'sec-ondary arthrocele and its arthritis index score.In this way,the arthritis symptom of AA rat is relieved,the levels of TNF -αand IL -1 in serum are reduced.In a word,compared with using sinomenine alone,the medicine combination of sinomenine and methotrexate has better therapeutical effect on RA.
