Metabonomic study of traditional Chinese herb pair, Qinghao-Biejia in treating systemic lupus erythematosus mice
10.3969/j.issn.1001-1978.2016.05.026
- VernacularTitle:青蒿-鳖甲药对配伍治疗系统性红斑狼疮小鼠的代谢组学研究
- Author:
Juan CHEN
;
Jun DENG
;
Jia ZHOU
;
Zhijun XIE
;
Jing GAN
;
Chengping WEN
- Publication Type:Journal Article
- Keywords:
traditional Chinese herb pair,Qinghao-Biejia;
systemic lupus erythematosus;
mice;
serum;
metabonomics;
high performance liquid chromatogra-phy coupled with Q-TOF/MS
- From:
Chinese Pharmacological Bulletin
2016;32(5):727-731,732
- CountryChina
- Language:Chinese
-
Abstract:
Aims To study the changes of serum me-tabolites in systemic lupus erythematosus ( SLE ) mice ( MRL/lpr) by treatment of Qinghao-Biejia and to ex-plore the pathogenesis of SLE and mechanism of drug action. Methods The serum samples of control group, SLE model group and Qinghao-Biejia treatment group ( low and high dose ) were collected, the metabolic profile of samples was analyzed by high performance liquid chromatography-quadrupole-time of flight mass spectrometry system ( HPLC-Q-TOF/MS ) . Software of Mass Hunter and Mass Profiler Professional ( MPP ) were used to process the data. A supervised mode of partial least squares-discriminant analysis ( PLS-DA ) was applied to recognize the data pattern. Results There were obvious disorders of lipid metabolism in SLE model. Compared with control group, Qinghao-Biejia treatment group improved lipid metabolism, af-fected the thrombosis development of SLE; and Qing-hao-Biejia treatment group reduced the pathological damage by improving inflammatory acute phase of SLE in mice. Conclusion Qinghao-Biejia treatment plays a therapeutic role in repairing the imbalance by multidi-mensional metabolic pathways in SLE mice.
