Effects of dietary high-salt intake on fibrosis and expression of microRNA133a in Dahl S rats’myocardium
10.7652/jdyxb201603013
- VernacularTitle:高盐干预对盐敏感高血压大鼠心脏microRNA133a表达及纤维化的影响
- Author:
Jie ZHANG
;
Jing WANG
;
Fuqiang LIU
;
Jianjun MU
- Publication Type:Journal Article
- Keywords:
salt;
salt sensitivity rats;
heart;
microRNA133a;
fibrosis;
hypertension
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2016;37(3):363-366,371
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of high-salt intake on the blood pressure,weight of left ventricle,myocardial microRNA133a and fibrosis levels by implementing the high-salt diet intervention trial in Dahl salt-sensitive rats and SD rats so as to explore the role of microRNA133a in salt-sensitive hypertensive cardiac fibrosis with high-salt intervention.Methods Sixteen 4-week male Dahl salt-sensitive rats are divided into two groups,8 in the SLS group and 8 in the SHS group.Similarly,sixteen 4-week male SD rats were divided into two groups. Systolic blood pressure of the rats was measured by the indirect tail-cuff method.Left ventricular mass index was measured after the rats were sacrificed. The expression levels of left ventricular myocardial tissue collagen I (LVMI),connective tissue growth factor (CTGF)expression and microRNA133a were observed.Results Blood pressure in high-salt groups both increased (P<0.01).Compared with that of SD rats,blood pressure was more significantly increased in high-salt group than in low-salt group of Dahl salt-sensitive rats (P<0.01).After the intervention of high-salt diet,LVMI,CTGF and collagen I expression in Dahl salt-sensitive rats increased more significantly than the low-salt group,with a significant difference (P<0.01);however,there was no significant difference between high-salt and low-salt groups (P>0.05).After the intervention,microRNA133a expression was significantly decreased in all high-salt groups compared with that in low-salt groups (P<0 .0 1 ),and the expression decreased more significantly in high-salt group of Dahl salt-sensitive rats than in high-salt group of SD rats (P<0.05).Conclusion High-salt diet is probably involved in salt-sensitive hypertension myocardial fibrosis by downregulating the expression of myocardial microRNA133a.
