TNF-αmediated activation of IDO pathway involved in mechanism of depression in rats induced by CUS
10.3969/j.issn.1001-1978.2016.05.007
- VernacularTitle:CUS致大鼠抑郁行为涉及TNF-α及IDO-HAAO通路激活
- Author:
Xiaoyan FU
;
Haiyan LI
;
Ting CUI
;
Qingsong JIANG
;
Hongmei QIU
- Publication Type:Journal Article
- Keywords:
depression;
CUS;
TNF-α;
IDO;
HAAO;
cytokines
- From:
Chinese Pharmacological Bulletin
2016;32(5):620-624,625
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the relationship be-tween the depression-like behaviors of rats induced by chronically unpredictable stress( CUS) and the protein expression of tumor necrosis factor alpha( TNF-α) , in-dole-2 , 3-dioxygenase ( IDO ) and 3-hydroxyl amino acid oxygenase( HAAO) . Methods Thirty male Spra-gue Dawley( SD) rats were randomly divided into con-trol group and model group. CUS plus solitary condi-tion were used to establish the depression model. The open field test ( OFT ) and the force swimming test ( FST ) were used to evaluate the depression-like behaviors of rats ;Western blot was used to determine the protein expressions of TNF-α, IDO and HAAO in the prefrontal cortex and hippocampus of rats. Results Model group rats showed obvious depression-like be-haviors with increasing immobile time in FST ( P <0. 01) and decreasing locomotive activity in OFT( P<0. 01 ) , and up-regulating the protein expression of TNF-α, IDO and HAAO in the prefrontal cortex and hippocampus significantly ( P <0. 05 ) , compared with control group rats. Conclusion The depression-like behaviors of rats induced by CUS may be associated with the activation of IDO-HAAO pathway mediated by TNF-α.
