Effect of Hedgehog pathway on myocardial infarction and hypoxic-ischemic cardiomyocytes
10.3969/j.issn.1001-1978.2016.05.016
- VernacularTitle:Hedgehog通路对心肌梗死及心肌细胞缺血缺氧的作用机制研究
- Author:
Yao CHEN
;
Xiyong YU
- Publication Type:Journal Article
- Keywords:
Hedgehog pathway;
myocardial infarc-tion;
ischemia;
hypoxia;
H2 O2;
myocardial cells
- From:
Chinese Pharmacological Bulletin
2016;32(5):676-680,681
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of Sonic Hedgehog on normal hearts and hypoxic-ischemic myo-cardial cells. Methods A method for left anterior de-scending artery ( LAD ) ligation was employed to con-struct the myocardial infarction model, and ultrasonic cardiogram was used for identification. Western blot and immunofluorescence staining were used to detect expressions of Shh, Ptch-1, Smo and Gli-1 in H9C2 cells and H2 O2-induced H9C2 cells, and that in 12 ca-ses of myocardial infarction tissues and 9 cases of nor-mal myocardium, respectively. Agonist and antagonist of Shh pathway were adminstered in the hypoxic-ische-mic myocardial H2 O2-induced H9C2 cell model, and once again expressions and strength of Shh, Ptch-1, Smo, Gli-1 were detected. Results Shh and Gli-1 were not expressed in normal hearts, but expressed in hearts with myocardial infarction;Ptch-1 and Smo were expressed in both normal hearts and hearts with myo-cardial infarction. Under the action of agonist, expres-sions of Shh and Gli-1 increased in the hypoxic-ische-mic H9C2 cell model. Similarly, Shh and Gli-1 were not expressed in normal H9C2 cells, but in H2 O2-in-duced H9C2 cells, and Ptch-1 and Smo were expressed in both normal H9C2 and in H2 O2-induced H9C2 cells. Conclusion Shh signaling pathway can be acti-vated in the condition of ischemia and oxidative stress, and then it promotes the repairing of myocardial cell damage.
