Effects of high insulin on chemotherapeutic sensitivity in MCF-7/ADR cells
10.11958/20150257
- VernacularTitle:高胰岛素对MCF-7/ADR细胞化疗敏感性的影响
- Author:
Nannan ZHU
;
Lan MU
;
Daiqing LI
- Publication Type:Journal Article
- Keywords:
insulin;
P-glycoprotein;
drug resistance,neoplasm;
Epirubicin;
MCF-7/ADR cell
- From:
Tianjin Medical Journal
2016;44(5):560-562,563
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse the effects of high insulin on the expression and function of P-glycoprotein (P-gp), and preliminarily investigate the influence of insulin on chemotherapeutic sensitivity in MCF-7/ADR cells. Methods MCF-7/ADR cells were cultured with different concentrations of insulin(0.001, 0.005, 0.01, 0.05 and 0.1μmol/L). Real-time PCR was used to detect the expression of P-gp mRNA. Western blot assay was used to detect the expression level of P-gp. Rhodamine 123 was used to detect the efflux function level of P-gp. Cell viability and chemotherapeutic sensitivity were detected by MTT assay. Results High concentration of insulin (0.1 μmol/L) promoted the proliferation of MCF-7/ADR cells. The concentration of insulin (0.05 and 0.1 μmol/L) accelerated P-gp mRNA and protein expression, which also augmented the efflux function of P-glycoprotein and reduced the chemotherapeutic sensitivity to epirubicin. Conclusion High concentration of insulin may influence the drug resistance of breast cancer cells by promoting the expression and function of P-glycoprotein of MCF-7/ADR cells.
