Study on pharmacological effects and mechanism of Naozhifu as a new drug candidate for prevention and treatment of Alzheimer′s disease base on multiple targets strategy
10.3969/j.issn.1671-8348.2016.10.005
- VernacularTitle:基于多靶点防治AD策略的候选新药脑智复的药理作用及其机制研究
- Author:
Jiachen AI
;
Jingyue XIONG
- Publication Type:Journal Article
- Keywords:
Alzheimer′s disease;
Naozhifu;
Aβ1-42;
ibotenic acid
- From:
Chongqing Medicine
2016;45(10):1313-1315,1319
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of Naozhifu as a multifunctional agents for the prevention and treatment of Alzheimer’s disease(AD) .Methods On 30th after castration ,female mice were received intrahippocampal injection of Aβ1‐42 and ibotenic acid for replicating the AD model .On the next day ,these modeled mice were randomly divided into model group ,low dose of Naazhifu group ,middle dose of Naozhifu group ,high dose of Naozhifu group and piracetam group ,sham operated mice were set as control group .Then the gavage was given once a day for 4 consecutive weeks .Furthermore the step‐down passive avoidance test , morris water maze test and biochemical indicators detection were performed .Results In step‐down passive avoidance test ,the latent period in model group was significantly shortened(P<0 .05) ,the fault frequency was increased when compared with control group (P<0 .05) .During the Morris Water Maze test ,the latent period for seeking escape from platform in AD mice were obviously ex‐tended(P<0 .05) ,the times of passing through the platform was shortened(P<0 .05) ,the swimming path length around the escape from platform ,swimming time and distance in the effective area in AD mice were increased(P<0 .05) ,meanwhile the level of MDA in brain tissue was significantly increased ,while the levels of Ach and SOD were significantly decreased(P<0 .05) .These indicators in middle and high dose of Naofuzhi group obviously improved .Conclusion Naofuzhi has the multi‐target prevention and treatment effect on AD .
