Establishment of canine spinal cord injury model and significance of laser-doppler flowmetry evaluation
10.3969/j.issn.1671-8348.2016.10.007
- VernacularTitle:脊髓损伤犬模型的建立及激光多普勒血流测定仪评价的意义
- Author:
Yujiang WU
- Publication Type:Journal Article
- Keywords:
laser-Doppler flowmetry;
spinal cord injury;
hemodynamics;
canine model
- From:
Chongqing Medicine
2016;45(10):1320-1322,1325
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of laser‐doppler flowmetry (LDF) in the evaluation of established canine spi‐nal cord injury(SCI) model .Methods Totally 15 beagle dogs were randomly divided into the group A ,B and C .Through the im‐proved numerical control SCI apparatus ,different impacts and compression time were adopted to establish the different acute SCI models .The group A was 1 .2 m/s+5 mm+5 mm+15 mns ,the group B was 1 .2 m/s+5 mm+5 mm+10 mns ,while the group C wastakenasthecontrolgroup.LDFwasusedtomeasurethespinalcordbloodflow(SCBF)chart,thepathologicchangewasevalu‐ated by measuring the spinal cord necrosis area .Results The SCBF chart in 3 groups measured by LDF showed that the decrease of SCBF in the group A was most obvious ,the injury degree in the group A was more severe than those in the group B and C ,the difference was statistically significant(P<0 .05) .The motor evoked potential displayed that the decrease degree of SCBF was close‐ly related with the prognosis and motion function .Conclusion LDF could effectively judge the degree of spinal injury and SCBF sit‐uation ,which has a good application prospect in animal experiments and clinical field .
