Antitumor effect of malaria genetically attenuated sporozoites infection in a murine lewis lung cancer model

VernacularTitle:疟原虫遗传减毒子孢子诱导抗肺癌免疫的初步研究
Author: Xufeng DENG ; Hong ZHENG ; Dong ZHOU ; Quanxing LIU ; Yan DING ; Wenyue XU ; Jigang DAI
Publication Type:Journal Article
Keywords: lung neoplasms; malaria; genetically attenuated sporozoites; lewis lung cancer
From: Chongqing Medicine 2016;45(11):1441-1443,1447
CountryChina
Language:Chinese
Abstract: Objective To learn whether plasmodium genetic attenuated sporozoites (GAS) can induce immunity against lung cancer ,in order to provide new ideas for the study of lung cancer vaccine .Methods Ther study was divided into two groups respec‐tively ,experimental group received intravenous injection of genetically attenuated sporozoites to immunize C57BL/6J mice and con‐trol group injection of phosphate buffer solution (PBS);after 14 days ,we subcutaneously inoculated lewis lung cancer (LLC) cells , calipers was used to measure tumor size .Immunohistochemical staining was detected tumor proliferation ,apoptosis ,and angiogene‐sis .Results There was statistically significant in tumor size .Immunohistochemical staining revealed that attenuated sporozoites in‐fection inhibited LLC eslls proliferation ,angiogenesis ,apoptosis .Conclusion The malaria attenuated sporozoites may provide a no‐vel strategy or therapeutic vaccine vector for anti‐lung cancer immune‐based therapy .