Effects of RASI on expression of airway MUC5 AC and related inflammatory factors in asthmatic mice with Yin deficiency syndrome
10.3969/j.issn.1000-484X.2016.01.009
- VernacularTitle:当归对阴虚哮喘小鼠气道MUC5 AC及相关炎症因子表达的影响
- Author:
Zhiwang WANG
;
Xiaoli CHENG
;
Yuan REN
;
Haiyan TUO
;
Xuefeng LIU
;
Rongke LI
- Publication Type:Journal Article
- Keywords:
Radix Angelicae Sinensis (RASI);
Asthma with Yin deficiency syndrome;
BALB/c mouse;
MUC5AC;
IL-13;
TNF-α
- From:
Chinese Journal of Immunology
2016;(1):42-45,50
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Radix Angelicae Sinensis ( RASI) on expression of airway MUC5AC and related inflammatory factors in asthmatic mice with Yin deficiency syndrome.Methods:Injecting ovalbumin ( OVA) to sensitize,inhaling OVA to stimulate,using Thyroxin during late stimulation,the asthmatic mouse with Yin deficiency syndrome was established and evaluated through asthmatic behaviors, lung histopathology, active factors ( IL-13, TNF-αand MUC5AC ) in broncho-alveolar lavage fluid (BALF), and MUC5AC expression in lung tissue.Results: 2,4,8 g/kg RASI could reduce asthmatic behaviors score, relieve pathological changes of lung tissue,reduce the contents of IL-13,TNF-αand MUC5AC in BALF,and depress MUC5AC expression in lung tissue ( P<0.05,0.01).In addition,there was a certain synergy between RASI and dexamethasone ( DXM) on depressing the ex-pression of IL-13 and MUC5AC (P<0.05).Conclusion:RASI has certain anti-asthma effect and one of mechanisms is to regulate the MUC5AC expression through inhibit IL-13 and TNF-α.On the expression of IL-13 and MUC5AC,the compatibility of RASI with glu-cocorticoid has some synergy effect.
