Effect of alum on immune response in mice induced by HBoV1 VP2 VLPs
10.3969/j.issn.1000-484X.2016.01.012
- VernacularTitle:铝佐剂对HBoV1 VP2 VLPs诱导小鼠免疫应答的影响
- Author:
Zhonghua DENG
;
Zhaojun DUAN
;
Zhiping XIE
;
Leyun XIE
;
Bing ZHANG
;
Youde CAO
- Publication Type:Journal Article
- Keywords:
Alum;
Human bocavirus-1(HBoV1);
Viral protein 2(VP2);
Virus-like particles(VLPs)
- From:
Chinese Journal of Immunology
2016;(1):56-58,64
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of alum on immune response in mice induced by HBoV1 VP2 VLPs.Methods:BABL/c mice were randomly divided into VLPs experimental group, alum adjuvant experimental group, PBS control group and alum adjuvant control group,the experimental group mice were intramuscular immunization with HBoV1 VP2 VLPs and HBoV1 VP2 VLPs added alum,control group mice were immunization with alum or PBS buffer,then to study the effect of alum on immune response in mice induced by HBoV1 VP2 VLPs by cellular and humoral immune strength.Results: Alum adjuvant decreased cellular immune response induced by HBoV1 VP2 VLPs(P<0.001),enhance the HBoV1 VP2 VLPs immuned serum IgG titer(P<0.05)and IgG activity(P<0.01).Conclusion:Alum adjuvant can enhance humoral immune response induced by HBoV1 VP2 VLPs,but weaken cellular immune response induced by HBoV1 VP2 VLPs,when HBoV1 VP2 VLPs used as a prophylactic vaccine it should add alum adjuvant,when used as a therapeutic vaccine,it should not add alum adjuvant.
