Expression of transcription factor BACH2 and its significance on PBMC in patients with coronary artery disease
10.3969/j.issn.1000-484X.2016.01.015
- VernacularTitle:转录因子BACH2在冠心病患者外周血单个核细胞中的表达及意义
- Author:
Shengshuai SHAN
;
Boyuan WANG
;
Shan KAI
;
Jibin LIN
;
Jing LIN
;
Dazhu LI
- Publication Type:Journal Article
- Keywords:
Coronary artery disease;
Transcription factor BACH2;
TLR4;
Inflammatory cytokines
- From:
Chinese Journal of Immunology
2016;(1):69-73,78
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of transcription factor BACH2 on PBMC in patients with coronary artery disease( CAD) and discuss its significance and role.Methods:The circulating levels of BACH2 and TLR4 in PBMC were evaluated by RT-PCR and Western blot separately.Serum levels of signature cytokines of T-helper cells,cytokines and ox-LDL were evaluated by ELISA.The data among groups were analyzed by one-way ANOVA.Correlation between variables were subjected to linear regression analysis.Results:The expression level of BACH2 down-regulated and TLR4 up-regulated significantly in PBMCs of ACS patients had significantly higher concentrations of serum ox-LDL,cytokines of TH1 cells,TH2 cells and TH17 cells and low level of cytokine of Treg cells.Negative correlation exists significantly between BACH2 and TLR4 in patients with CAD and between BACH2 and IFN-γ,IL-4 and IL-17,and between BACH2 and ox-LDL in serum.However, positive correlation exists significantly between BACH2 and TGF-β1.Conclusion:Increased serum concentrations of ox-LDL probably down-regulated the expression of BACH2 in ACS patients by activating TLR4 on PBMC, resulting in promoting production of pro-inflammatory cytokines and suppressing production of anti-inflammatory cytokine and then might cause ACS.
