Observation on growth status of Blastocystis hominis in three media
10.16250/j.32.1374.2015163
- VernacularTitle:3种培养基中芽囊原虫生长状况观察
- Author:
Yingfang YU
;
Xiuping WU
;
Yanhong CHU
;
Jiaxu CHEN
;
Liguang TIAN
- Publication Type:Journal Article
- Keywords:
Blastocystis hominis;
Culture in vitro;
Growth condition;
Morphology observation;
Medium;
Jone’s;
Vitro;
1640
- From:
Chinese Journal of Schistosomiasis Control
2016;(1):51-53,65
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the growth situation of Blastocystis hominis in vitro and select the optimal method for culti?vation of B. hominis in different media. Methods Ten positive stools with B. hominis were inoculated in three different media for cultivating,namely 1640,Jone’s medium and vitro medium. And the stools with good growth status and high quantities of B. hominis were chosen to inoculate in the three media with equal amount after subcultivation,and the number of B. hominis was counted every 24 h for ten days,and the morphological changes and growth status were also observed. Results The densities of B. hominis in the 1640 and Jone’s medium were higher than that in the vitro medium 48 h after the inoculation. The same stool sample was inoculated to the three different media and observed for ten days,and the results indicated that the growth of B. homi?nis presented regular changes in the three media,the growth peaks were on the third,sixth and ninth day post inoculation;and the density of B. hominis was the highest in the Jone’s medium. The morphology of B. hominis was the clearest and most dynamic in the vitro medium,while various reproductive forms were observed in the Jone’s medium. Conclusion Jone’s medium is suitable for the growth of B. hominis and can be the first choice for the cultivation of B. hominis in vitro,and vitro medium is the best medium for observing the growth situation of B. hominis.
