Imbalance of Th1/Th2 in patients with systemic lupus erythematosus combined with coronary heart disease
10.3969/j.issn.1671-8348.2016.02.022
- VernacularTitle:系统性红斑狼疮合并冠状动脉性心脏病患者Th1／Th2失衡的研究
- Author:
Shan ZHAO
;
Juan YU
;
Hong ZHANG
- Publication Type:Journal Article
- Keywords:
erythematosus,systemic;
coronary disease;
T-lymphocytes,helper-inducer;
Th1;
Th2
- From:
Chongqing Medicine
2016;(2):216-218,222
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the imbalance of Th1/Th2 cell response in patients with systemic lupus erythematosus (SLE) combined with coronary heart disease .Methods SLE patients ,SLE patients with coronary heart disease and healthy con-trols were enrolled and blood samples were collected .T-bet/GATA-3 ,the transcription factors of Th1/Th2 cells ,were detected by real-time PCR ;the intracellular cytokines IFN-γ and IL-4 in CD4 + T cells were stained by fluorescent antibodies and detected by flow cytometry ;the level of serum IFN-γ and IL-4 were detected by ELISA .Results Comparing with healthy control group ,the ex-pression level of Th1 transcription factor T-bet ,the introcellular secretion of IFN-γ in CD4 + T cells and the serum IFN-γ were all decreased in non-coronary heart disease patients with SLE( P < 0 .05) .Comparing with non-coronary heart disease patients with SLE or healthy control group ,the expression level of Th1 transcription factor T-bet ,the introcellular secretion of IFN-γ in CD4 + T cells and the serum IFN-γ were all increased in patients with SLE combined coronary heart disease(P< 0 .05) ;while the expression level of Th2 transcription factor GATA-3 ,the introcellular secretion of IL-4 in CD4 + T cells and the serum IL-4 were all decreased in patients with SLE combined coronary heart disease(P< 0 .05) .Conclusion There were imbalance towards Th1 cell response in patients with SLE combined coronary heart disease ,which may related to the occurrence and development of disease .
