Effects of Shenqi Bufei Tang Decoction on the Expression of HDAC2 and NF-κB p65 in Airway Smooth Muscle Tissues of COPD Rats with Lung-Qi Deficiency Syndrome
10.3870/j.issn.1004-0781.2016.03.002
- VernacularTitle:参芪补肺汤对COP D肺气虚证大鼠气道平滑肌HDAC2与NF-κB p65表达的影响?
- Author:
Guiying WU
;
Kui ZHANG
;
Ping YAN
;
Xiangyan ZHANG
;
Sha LUO
;
Lili SHEN
;
Zhu YANG
- Publication Type:Journal Article
- Keywords:
Shenqi Bufei Tang decoction;
Pulmonary disease,obstructive,chronic;
Smooth muscle,airway;
Histone deacetylase-2;
Nuclear factor -κB p65
- From:
Herald of Medicine
2016;(3):224-228,229
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effects of Shenqi Bufei Tang decoction on the expression of histone deacetylase-2 ( HDAC2) and nuclear factor-κB p65 ( NF-κB p65) in the airway smooth muscle tissues of COPD rats with lung-qi deficiency syndrome. Methods A total of 40 male rats were randomly divided into normal control group,model control group,Shenqi Bufei Tang decoction group,and aminophylline group.The COPD rat model with lung-qi deficiency syndrome was established by intra-tracheal instillation of lipopolysaccharide (LPS) and passive smoking for 28 days.Pathological changes of lung tissues were ob-served under the light microscope and the thickness of the small airway wall and airway smooth muscle ( ASM) layer analyzed by the image analysis.Immunohistochemistry,real-time PCR and Western blotting were used to detect the expressions of NF-κB p65 and HDAC2 in ASM. Results The thickness of the airway wall and ASM,and the expression levels of NF-κB p65 mRNA and protein were significantly increased in the model control group when compared with those in the normal control group ( P<0.05) . They were significantly reduced and the expression levels of HDAC2 mRNA and protein were markedly increased in Shenqi Bufei Tang decoction group and aminophylline group,which were compared with those in the model group (P<0.05).There were no sig-nificant differences in these indices between Shenqi Bufei Tang decoction group and aminophylline group (P>0.05). Conclu-sion Shenqi Bufei Tang decoction can inhibit the proliferation of ASM in COPD rats with lung-qi deficiency syndrome,which may be associated with the increased expression of HDAC2 and decreased expression of NF-κB p65.
