Expression of bone morphology proteins in mice with collagen-induced arthritis
10.3969/j.issn.1671-8348.2016.03.002
- VernacularTitle:骨形态发生蛋白在胶原诱导关节炎小鼠模型中的表达
- Author:
Qiang LIU
;
Yingying ZHANG
;
Zhijun JIAO
;
Jinjin GU
- Publication Type:Journal Article
- Keywords:
bone morphogenetic proteins;
polymerase chain reaction;
CIA mice
- From:
Chongqing Medicine
2016;(3):293-295,298
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish collagen‐induced arthritis (CIA) model used of DBA/1J mouse ,a preliminary study of the expression levels of BM Ps in mononuclear cells of peripheral blood and the joints tissue of CIA mice .Methods We induced DBA/1J mice and developed arthritis pathology by using of bovine type Ⅱ ;then ,we detected mRNA and protein expression levels of BMPs by using quantitative PCR and immunohistochemical staining .Results We successfully established CIA mouse model .The expres‐sion of BMP4 and BMP9 mRNA was significantly down‐regulated in peripheral blood mononuclear cells and in the pathogenesis of joint tissues of CIA mice (P<0 .05) .It showed that BMP9 protein significantly decreased in joint tissues of CIA mice by immuno‐fluorescence technique (P=0 .002) .Conclusion At the genetic level ,the expression of BMP4 and BMP9 could be significantly down‐regulated in the CIA mouse .At the protein level ,BMP9 could be significantly down‐regulated in the CIA mouse .
