Establishment renal calculus rat model induced by calcifying nanoparticles
10.3969/j.issn.1671-8348.2016.03.007
- VernacularTitle:钙化性纳米微粒致大鼠肾结石模型的构建
- Author:
Jie WANG
;
Juan WANG
- Publication Type:Journal Article
- Keywords:
nephrolithiasis;
calcinosis;
lens,crystalline;
calcifying nanoparticles;
model;
shenshitong;
gallium nitrate
- From:
Chongqing Medicine
2016;(3):310-312,316
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish renal calculus rat model induced by calcifying nanoparticles .Methods We extracted and cultivated calcifying nanoparticles from clinically diagnosed patients with nephrolithiasis .Forty adult male SD rats were divided into 4 groups (n=10 each):group A(given an intravenous injection of calcifying nanoparticles) ,group B(given an intravenous injection of calcifying nanoparticles and taken Shenshitong granules) ,group C(given an intravenous injection of calcifying nanoparticles and intraperitoneal injection of gallium nitrate) ,group D (as normal control) .Eight weeks later ,all rats were sacrificed .The kidneys were examined for pathology .Results Histopathological studies revealed calcifying nanoparticles induced renal tubular epithelial cells fatty degeneration and renal tubular crystallization ,and caused renal interstitial infiltration with inflammatory cells .The forma‐tion of renal crystals in group A was more obvious than those in group B and C(P<0 .05) .In group D ,no crystals were formed and no pathological changes occurred .The levels of urinary calcium and urinary creatinine(of All group) at one day before the observa‐tion period (T2 ) were higher than those at 4 weeks after injection(T1 )(P<0 .01) .The levels of urinary calcium and serum creati‐nine in group A and B and C were significantly higher than those in group D at T2 (P<0 .05) .The levels of urinary calcium in group B and C were lower than that in group A at T2 (P<0 .05) .However ,there were no apparent differences of the levels of serum calci‐um and urinary creatinine between groups at T2 (P>0 .05) .Conclusion Calcifying nanoparticles could cause renal tubular epithelial cells injury ,which leads to the formation of kidney stones .
