Study on Influencing Factors for Proliferation and Differentiation of Dendrobium officinale Protocorm-like Body
10.13359/j.cnki.gzxbtcm.2015.06.027
- VernacularTitle:铁皮石斛原球茎增殖与分化影响因素研究
- Author:
Yingya XU
;
Yan WANG
;
Hongyuan LIU
;
Xiaoping LAI
;
Guifang ZHANG
- Publication Type:Journal Article
- Keywords:
Dendrobium officinale Kimura et Migo/growth&development;
Protocorm-like body;
Proliferation;
Differentiation
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2015;32(6):1091-1097,1122
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the optimal medium formula for obtaining high proliferation rate and differentiation rate of protocorm-like body ( PLB) of Dendrobium officinale Kimura et Migo ( D.officinale) . Methods We observed the effect of different concentrations of phytohormones such as 6-benzylaminopurine (6-BA) , 1-naphthaleneacetic acid ( NAA) , 2,4-dichlorophenoxyacetic acid ( 2,4-D) and kinetin ( KT) , or their combinations together with organic additives such as potato extractive ( PE) , banana extractive ( BE) , apple extractive ( AE) and coconut milk ( CM) on the proliferation and differentiation of PLB of Dendrobium officinale Kimura et Migo. Results 6-BA, NAA and KT at certain concentrations were beneficial to the proliferation and growth of PLB, and when the concentration of KT was 1.0 mg/L, 50-d PLB proliferation times reached to 7.20. 2,4-D had obvious inhibitory effect on PLB proliferation and growth. The combination of 6-BA, NAA and KT showed stronger effect on PLB proliferation than the phytohormones used alone, and the combination of 0.5 mg/L 6-BA, 1.0 mg/L KT and 1.0 mg/L NAA had the best effect on PLB proliferation, 50-d PLB proliferation times reaching to 9.52. Certain concentrations of PE and AE could promote PLB differentiation, and 100 g/L PE brought well-grown seedling in test tube and the highest differentiation rate, being up to 92.6% . Conclusion The optimal hormone matching and additives concentration on proliferation and differentiation of PLB of Dendrobium officinale Kimura et Migo have been obtained, which can lay a foundation for its rapid propagation and artificial seed preparation.
