Determination of Emodin and Danshensu in Pinggan Oral Liquid by HPLC
- VernacularTitle:HPLC测定平肝口服液中大黄素和单参素的含量
- Author:
Yang WU
;
Xu SUN
;
Yuan GAO
;
Xingdong LI
;
Xiaoyu MA
;
Huifeng YAO
;
Yao CHEN
- Publication Type:Journal Article
- Keywords:
Pinggan oral liquid;
Emodin;
Danshensu;
HPLC
- From:
China Pharmacist
2016;19(4):758-760,761
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the method for the determination of emodin and danshensu Pinggan oral liquid. Methods:The contents were determined by HPLC. The chromatographic conditions for emodin were as follows: an Agilent Eclipse XDB-C18 column (150 mm × 4. 6 mm, 5μm) was used and the mobile phase was methanol-water (80 ∶20). The detection wavelength was 287 nm and the flow rate was 1. 0 ml·min-1 . The column temperature was 25℃ and the injection volume was 20 μl. The chromatographic condi-tions for Danshensu were as follows:an Agilent Eclipse SB-C18 column (150 mm × 4. 6 mm, 5 μm) was used and the mobile phase was methanol-1% acetic acid solution (2 ∶98). The detection wavelength was 280 nm and the flow was 1. 0 ml·min-1. The column temperature was 30℃ and the injection volume was 20 μl. Results:Emodin had a good linear relationship within the range of 26. 75-428. 00 μg·ml-1(r=0. 999 9). The average recovery was 102. 7% and RSD was 0. 76% (n=6). Sodium Danshensu had a good linear relationship within the range of 10.17-162.77 μg·ml-1(r =0.999 9). The average recovery was 100.5% and RSD was 1. 47% (n=6). Conclusion:The method is simple, accurate and reproducible, which can be used for the quality control of the prep-aration.
