Effect of Rat Intestinal Bacteria on Metabolism of Pedunculoside in vitro
- VernacularTitle:大鼠肠道菌群对长梗冬青苷体外代谢的影响
- Author:
Di CAO
;
Zhen FAN
;
Jinping ZHU
;
Bao YANG
;
Lian ZHOU
;
Jing JIN
;
Zhongxiang ZHAO
- Publication Type:Journal Article
- Keywords:
Rat intestinal bacteria;
Pedunculoside;
Rotundic acid;
HPLC
- From:
China Pharmacist
2016;19(4):621-623,624
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the metabolism of pedunculoside treated with rat intestinal bacteria in vitro. Methods:Pedunculo-side and rat intestinal bacteria were incubated in vitro for 0, 4, 8, 24 and 48 hours under anaerobic condition. After extracted repeat-edly by ethyl acetate, the metabolites in the incubation media were qualitatively and quantitatively analyzed by HPLC. Results:Totally 90. 8% of pedunculoside was transformed to M2 after incubated with rat intestinal bacteria in vitro for 48 hours, and a detailed compari-son of HPLC profiles between M2 and rotundic acid showed M2 was rotundic acid. Conclusion: Pedunculoside can be metabolized to rotundic acid by rat intestinal bacteria in vitro.
