Content Comparison of Specnuezhenide in Traditional Decoction and Granules of Zishenwufa Formula
- VernacularTitle:滋肾乌发方传统煎剂与配方颗粒中特女贞苷含量的比较
- Author:
Meng WANG
;
Xujie ZANG
;
Ye REN
- Publication Type:Journal Article
- Keywords:
Zishenwufa formula;
Specnuezhenide;
Traditional decoction;
Granules;
HPLC;
Content determination
- From:
China Pharmacist
2016;19(4):786-787,788
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of specnuezhenide in traditional decoction and granules of Zish-enwufa formula, and compare the content between them. Methods:HPLC was used for the determination. The samples were analyzed on a Waters Symmetry C18 column (150 mm × 3. 9 mm,5 μm) with acetonitrile-water (16 ∶84) as the mobile phase. The flow rate was 1. 0 ml·min-1 and the detection wavelength was 224nm. Results: A good linearity of specnuezhenide was within the range of 0.179 2-3.316 μg(r=0.999 9),and the average recovery of berberine hydrochloride was 101.65% with RSD of 1.49%(n=6). Conclusion:The determination method is simple, accurate and sensitive with good reproducibility,which can be used for the determi-nation of specnuezhenide in the preparation. The content of specnuezhenide in the traditional decoction and granules of Zishenwufa for-mula is similar.
