Detection and identification of a new bat bocavirus species in Yunnan

VernacularTitle:云南一种新型蝙蝠博卡病毒的鉴定与分析
Author: Xingyu LI ; Gang CHEN ; Lele XIA ; Weiguo ZHOU ; Lin XU ; Jing YU ; Wei QIU ; Fuqiang ZHANG ; Quanshui FAN ; Changchun TU ; Tingsong HU ; Biao HE
Publication Type:Journal Article
Keywords: Yunnan; bats; bocavirus; phylogenetic analysis
From: Military Medical Sciences 2016;40(3):207-212,233
CountryChina
Language:Chinese
Abstract: Objective To discover more novel bat viruses and molecularly characterize bat-borne bocavirus diversity in Yunnan.Methods Twenty-six Aselliscus stoliczkanus were sampled in Jinghong , Yunnan, and subjected to viral metagenomic analysis.Specific PCR was used to detect any bocavirus in these samples based on the metagenomic result , while full genome was amplified and compared with other bocaviruses .Results and Conclusion Totally, 3 of the 26 (11.5%) bats were positive for bocavirus, the full genome of which contained 5203 nucleotides and could encode NS1, NP and VP1/VP2 proteins.Phylogenetic analysis showed that this virus shared up to 58.7% and 53.3% amino acid identities with canine bocavirus 1 and canine minute virus .According to ICTV criteria (85%amino acid sequence identity ) on a new species of bocavirus , this virus could be a novel species within genus Bocaparvovirus .This study provides important data to better understand viral diversity in bats and to uncover the relationship between bocavirus and its hosts .