Induction of macrophages differentiation to M2 type by adipose derived mesenchymal stem cells

VernacularTitle:脂肪间充质干细胞诱导巨噬细胞向M2型极化的实验研究
Author: Lingling WANG ; Lili TANG ; Meng SUN ; Tianyang WANG ; Heyi YOU ; Chunwu ZHANG ; Yirong YANG ; Jicai CHEN ; Mengtao ZHOU ; Bicheng CHEN
Publication Type:Journal Article
Keywords: Adipose derived mesenchymal stem cells; Macrophages; RAW264.7; Differentiation
From: Chinese Journal of Immunology 2016;32(3):332-335,344
CountryChina
Language:Chinese
Abstract: Objective:To explore the effect of adipose derived mesenchymal stem cells to regulate the differentiation of macrophage RAW264.7.Methods:First,we used RAW264.7 cells to simulate macrophage and induced them to M 1 macrophage with lipopolysaccharide ( LPS,1 μg/ml) .Then we cultured these RAW264.7 cells in culture mediums which were previously used to culture adipose derived mesenchymal stem cells to imitate the transplantation of ADMSC .Last,the mRNA relative expression of IL-10, IGF-1,Arg-1,TNF-α,FIZZ1,SPHK-1 was detected by real-time PCR.The protein expression of IL-12 p40,IL-27 Rα,IL-10 was detected by Western blot.Results:After been cultured in ADMSCCM and induced by LPS ,M1 markers (TNF-αmRNA,IL-12 p40;P<0.05) of the RAW264.7 cells declined while M2 markers (IGF-1 mRNA,IL-10 mRNA,IL-10;P<0.05) rose.Conclusion: ADMSC can secrete soluble cytokines to induce the RAW264.7 cell,which have been induced to the M1 macrophages,to differentiate towards M2 macrophages.