TSLP promotes recruitment of myofibroblasts into airway in asthmatic mice
10.3969/j.issn.1000-484X.2016.06.002
- VernacularTitle:TSLP促进肌成纤维细胞向哮喘小鼠气道募集
- Author:
Ping MENG
;
Zhuanggui CHEN
;
Tiantuo ZHANG
;
Hongtao LI
;
Xiaoling ZOU
;
Hailing YANG
- Publication Type:Journal Article
- Keywords:
TSLP;
Myofibroblast;
Asthma;
Airway remodeling
- From:
Chinese Journal of Immunology
2016;32(6):777-781,786
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether thymic stromal lymphopoietin ( TSLP) participate in asthmatic airway remodeling partially by promoting myofibroblast accumulating in the lung. Methods:Twelve mice evenly were randomly divided into four groups:a saline group;an HDM-exposed group;an IgG isotype-treated group and an anti-TSLP-treated group. The supernatant of bronchoalveolar lavage fluid ( BALF) was used to analyze the levels of TSLP,IL-25 and IL-33 by ELISA. Fluorescence-labeled collagenⅠ( ColⅠ)/α-smooth muscle actin (α-SMA) -dual-positive myofibroblasts were examined by confocal microscopy. Results:Chronic allergen exposure induced obviously abnormal airway structural changes,which were inhibited by blocking TSLP. We detected a highly increased number of myofibroblasts in the sub-epithelial zone in mice from HDM-challenged group. However, TSLP neutralization significantly reduced myofibroblasts recruitment. Moreover,blocking TSLP not only decreased the level of TSLP,but also inhibited the expression levels of TGF-β1 and IL-33 in BAL fluid. Conclusion:The results suggest that orchestrating myofibroblasts recruiting into the lungs is one of the main pathogenesis that TSLP involves in airway remodeling in asthmatic mice.
