Effect of IL-21 on Treg cells in peripheral blood mononuclear cells in pathogenesis of Graves disease
10.3969/j.issn.1000-484X.2016.06.018
- VernacularTitle:IL-21调节外周血中Treg细胞的表达在Graves病发病机制中的研究
- Author:
Xiaoqing HUANG
;
Chun LIU
- Publication Type:Journal Article
- Keywords:
IL-21;
Graves disease;
Regulatory T cells;
Foxp3;
IL-10
- From:
Chinese Journal of Immunology
2016;32(6):853-857,862
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of IL-21 in regulating the expression of peripheral blood Treg cells in the pathogenesis of Graves disease ( GD ) . Methods: Electrochemical luminescence detection was used to determine levels of thyroid function indexes and autoantibodies. Peripheral blood mononuclear cells (PBMCs) were extracted,then,cultured in the presence or absence of IL-21 in vitro. The level of IL-10 protein was measured by enzyme-linked immunosorbent assay ( ELISA) ,and expressions of Foxp3 and IL-10 mRNA were examined by Real-time PCR. Results: Compared to eGD and control group, there were significant differences in the levels of thyroid function indexes in GD group (P<0. 05),whereas there was no difference between control group and eGD group (P>0. 05). Before IL-21 stimulation,compared to eGD and control groups,expressions of Foxp3 and IL-10 mRNA and level of IL-10 protein were significantly higher in GD group ( P<0. 05 );but there were no significant differences between eGD group and control group (P>0. 05). After IL-21 stimulation,expressions of Foxp3,IL-10 mRNA and IL-10 protein levels were decreased markedly in all three groups,among which GD group showed the greatest change(P<0. 05). Conclusion:IL-21 may inhibit the differentiation of Treg cells and the production of IL-10,therefore decreasing the number of Treg cells and the ability of suppressing effector T cells ,thus should be involved in the pathogenesis of GD.
