Observation of morphology and function of human parathyroid cells encapsulated by hydrogel
10.3760/cma.j.issn.1673-4203.2016.02.005
- VernacularTitle:水凝胶化成人甲状旁腺细胞形态和功能的观察
- Author:
Yue ZHAO
;
Bin LUO
- Publication Type:Journal Article
- Keywords:
Hydrogel;
Hypoparathyroidism;
Parathyroid hormone;
Feasibility studies
- From:
International Journal of Surgery
2016;43(2):85-87,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of the hydrogel as a cell carrier for parathyroid cells.Methods Cryopreserved parathyroid cells were recovered and cultured until sufficient,then the cells were digested、centrifuged and pipetted to single-cell suspension.The chitosan solution,crosslinking agents and cell suspension were mixed to hydrogels by some ratio.After that,hydrogels covered by complete medium were incubated at 37 ℃ in constant temperature incubator.Meanwhile,the supernatant were collected for measuring the PTH level every other day and the viability and shape of cells in hydrogels was also evaluated by fluorescopy.Results Parathyroid cells were recovered and cultured successfully and the adherent cells were spindle-like.The hydrogel could be prepared at room temperature in less than 1 minutes.The fluorescent confocal microscopy showed that more than 90％ of the cells in the hydrogel kept viable after 24 hours and the proportion had no significant change after 3 days.Furthermore,PTH was detected in the supernatants.Conclusion The facilely prepared inexpensive hydrogel,which can maintain normal morphlology and function of cells,is of potential application to cell transplantation therapy for hypopatathyroidism.
