Identification of molecular targets of radiotherapy in colorectal cancer
10.3760/cma.j.issn.1673-4181.2016.01.007
- VernacularTitle:放疗在大肠癌中作用分子靶标的筛选
- Author:
Yin LIANG
- Publication Type:Journal Article
- Keywords:
Gene expression profile;
Bioinformatics;
Colorectal cancer;
Radiotherapy
- From:
International Journal of Biomedical Engineering
2016;39(1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the potential therapeutic targets of radiotherapy in colorectal cancer via bioinformatics analysis of gene expression microarray.Methods The gene expression dataset GSE15781 was downloaded from the GEO database.The differentially expressed genes in radiotherapy treated colorectal cancer samples compared with those without radiotherapy,as well as differentially expressed genes in colorectal cancer samples without radiotherapy compared with normal colorectal tissues without radiotherapy,were obtained by R programming.The DAVID database was used for the functional enrichment analysis of the differentially expressed genes.Besides,protein-protein interaction (PPI) network of the overlapped genes between the two groups of differentially expressed genes were constructed based on the HPRD database.Results A total of 702 differentially expressed genes were obtained for the radiotherapy treated colorectal cancer samples compared with the ones without radiotherapy.Besides,126 differentially expressed genes in colorectal cancer samples without radiotherapy compared with normal colorectal tissues without radiotherapy were identified.Biological processes that related to cell adhesion,cell proliferation,as well as inflammatory response were found to be enriched in those differentially expressed genes.Moreover,16 overlaps were found between the two groups of differentially expressed genes,and in the PPI network of the 16 overlapped genes,PTGS2 and SDCBP2,etc,were closely correlated with other genes,which might be the important therapeutic targets of radiotherapy.Conclusions Some potential therapeutic targets of radiotherapy in colorectal cancer can be obtained by analysis of gene expression profiles,which may be very important for the study and treatment.
