Expression of SIRT3 protein in gastric carcinoma and its clinical significance
10.3760/cma.j.issn.1006-9801.2015.12.002
- VernacularTitle:SIRT3蛋白在胃癌中的表达及其临床意义
- Author:
Liming GAO
;
Liying KANG
;
Hongmei XU
;
Yuqing HE
;
Zhanzhao FU
;
Junli CAO
;
Ya DI
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
SIRT3;
Immunohistochemistry;
Western blot,protein
- From:
Cancer Research and Clinic
2015;27(12):798-800,809
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the correlation between SIRT3 protein and clinicopathological parameters of gastric carcinoma.Methods Immunohistochemistry and Western blot were used to detect the expression of SIRT3 in the gastric carcinoma and normal gastric tissue.The correlation between the expression of SIRT3 and clinicopathological parameters was analyzed.Results The immunohistochemistry showed that the positive expression rate of SIRT3 protein in gastric carcinoma tissue (53.8 ％,43/80) was obviously lower than that in normal gastric tissue (86.0 ％,43/50),and the expression of SIRT3 protein showed close relationship with invasion depth,lymph node metastasis and TNM stage (P ＜ 0.05),rather than the age,gender,tumor size,or differentiation status (P ＞ 0.05).The Western blot showed that the expression rate of SIRT3 protein (SIRT3/β-actin) in gastric carcinoma tissue (0.655±0.317) was lower than that in normal gastric tissue (0.803±0.329) (P ＜ 0.05).Conclusion The expression of SIRT3 protein is lower in gastric cancer than that in normal gastric tissue,and relates to invasion depth,lymph node metastasis and TNM stage.SIRT3 may inhibit the occurrence and development of gastric cancer.
