Expression and significance of MAGE-A9 in hepatocellular carcinoma
10.3760/cma.j.issn.1006-9801.2016.03.008
- VernacularTitle:MAGE-A9在肝细胞癌中的表达及其意义
- Author:
Maoying FU
;
Huihui NI
;
Wei ZHANG
;
Feng JIN
;
Li GONG
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocellular;
MAGE-A9;
Polymerase chain reaction;
Immunohistochemistry;
Prognosis
- From:
Cancer Research and Clinic
2016;28(3):174-178,182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of MAGE-A9 in human hepatocellular carcinoma (HCC), and to investigate the association between expression of MAGE-A9 and the clinicopathological characteristics of HCC.Methods Reverse transcription-polymerase chain reaction (RT-PCR), one-step quantitative-PCR (qPCR) and immunohistochemistry (IHC) methods were performed to characterize the expression of MAGE-A9 in HCC cell lines and tissues.Kaplan-Meier survival analysis and Cox regression were employed to evaluate the prognosis of 100 HCC patients.Results The expression of MAGE-A9 mRNA in HCC (4.44±0.342) was significantly higher than that in non-cancerous cells and tissues (1.73±0.178) (P ＜ 0.05).The expression level of the MAGE-A9 protein in HCC was related to the pathological grade (P =0.003), portal vein invasion (P =0.001), distant metastasis (P =0.022) and TNM stage (P =0.005).Cox regression analysis revealed that MAGE-A9 expression is an independent prognostic factor for disease-free survival (P =0.006) and overall survival (P =0.022).Conclusion MAGE-A9 is a valuable prognostic biomarker for HCC patients, and its high expression suggests unfavorable survival outcomes in HCC patients.
