Radiotherapy induced microRNA expression in the plasma of breast cancer patients
10.3760/cma.j.issn.0254-5098.2016.04.005
- VernacularTitle:放射治疗对乳腺癌患者血浆中microRNAs表达的影响
- Author:
Jia LIU
;
Gang GAO
;
Shikai WU
;
Chunnan PIAO
;
Jianxiang LIU
- Publication Type:Journal Article
- Keywords:
Ionizing radiation;
Breast cancer;
Radiotherapy;
miRNA;
Dose response
- From:
Chinese Journal of Radiological Medicine and Protection
2016;36(4):260-263,302
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of miR-210-3p,miR-221-3p,miR-21-5p and miR-150-Sp in the plasma of breast cancer patients before and after radiotherapy in order to establish reliable early biomarkers of non-uniform radiation injuries.Methods Blood samples were collected from 13 patients before radiotherapy (0 Gy) and 24 h after radiotherapy of 2,10,20,30 Gy.The miRNAs in the blood plasma were detected with qRT-PCR.Results The levels of miR-210-3p,miR-221-3p,miR-21-5p had no significant difference among different dosage groups after radiotherapy(P ＞ 0.05).There was no significant difference between the expression of miR-150-5p before and after 2 Gy radiotherapy (P ＞ 0.05).While the relative level of miR-150-5p gradually decreased to 0.808,0.605,0.565 (x2 =18.76,P ＜ 0.05) with increased accumulative dosage of 10,20,and 30 Gy,respectively.In addition,the miR-150-5p expression levels had no relationship (P ＞ 0.05) with situations (positive or negative group) of human epidermal growth factor receptor 2 (HER2),estrogen receptor (ER) and progesterone receptor (PR) in the breast cancer cells.Conclusions Ionizing radiation could reduce the expression of miR-150 in the plasma of breast cancer patients in a dose-dependent manner.
