The regulatory role of LiCl in microbial suppurative keratitis
10.3969/j.issn.1673-4130.2016.04.019
- VernacularTitle:氯化锂在细菌性化脓性角膜炎中的调控
- Author:
Kang CHEN
;
Qiang FU
;
Bing YI
;
Yanxiang LUO
;
Xiuming ZHANG
- Publication Type:Journal Article
- Keywords:
bacterial keratitis;
inflammation;
cell apoptosis
- From:
International Journal of Laboratory Medicine
2016;37(4):478-479,481
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of LiCl in microbial suppurative keratitis .Methods Western-blot assay was used to detect the efficacy of LiCl .Inflammatory cytokine levels were examined using Real-time PCR .Cell apoptosis was detected by using TUNEL and flow cytometry assays .Results LiCl promoted corneal resistance to PA infection .Real-time PCR data showed that LiCl enhanced IL-10 expression ,but suppressed TNF-expression .TUNEL and flow cytometry data showed that LiCl promoted the apoptosis of infiltrating cells .Conclusion LiCl promoted host resistance against microbial suppurative keratitis ,via regulating in-flammatory cytokine expression and cell apoptosis .
