Application of dilution regression method in the coagulation detection of fat blood samples
10.3969/j.issn.1673-4130.2016.04.026
- VernacularTitle:稀释回归法在脂血标本凝血检测中的应用探讨
- Author:
Chuanmei PENG
;
Yang WANG
;
Yunfang LIU
;
Min XU
;
Yun CHENG
;
Yanfeng WU
- Publication Type:Journal Article
- Keywords:
blood coagulation detection;
fat blood samples;
dilution regression method
- From:
International Journal of Laboratory Medicine
2016;37(4):494-495,498
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the dilution regression method of coagulation detection(PT ,APTT) in fat blood samples . Methods We collected 40 normal blood coagulation specimens (no fat blood ,no jaundice ,no hemolysis) in Yan′an hospital of Kun-ming ,then we detected the PT and APTT of the original plasma and 3-fold diluted plasma and 5-fold diluted plasma ,the we used the data both of before dilution and diluted to do the linear regression analysis ,and finally we got the regression equations of each index .we also collected 33 fat blood samples in Yanan hospital of Kunming ,which be divide into three groups through the severity of triglycerides :mild fat blood group(1 .7 mmol/L≤TG<11 .0 mmol/L) and moderate fat blood group(11 .0 mmol/L≤TG<20 .0 mmol/L)and severe fat blood group(TG≥20 .0 mmol/L) ,then we detected the PT and APTT after 3-fold diluted plasma and high-speed centrifugation plasma ,and then we brought diluted results into the normal regression equations and the results were compared with the high-speed centrifugation results .Results Because most of the 5-fold diluted plasma can not get the effective results ,so we use 3-fold diluted plasma to get the regression equations .The results of 3-fold diluted plasma was calculated by the regression equa-tions ,which then compared with high-speed centrifugation results ,after the analysis of statistical software ,two results had not sta-tistically significant (P>0 .05) .Conclusion Dilution regression method can be used to detect the fat blood samples in the clinical coagulation detection .
