Effects of phenytoin on VEGF and SCF in rat bone marrow mesenchymal stem cells and vascular endothelial cells co-culture system
10.7652/jdyxb201602012
- VernacularTitle:苯妥英钠对大鼠骨髓间充质干细胞与血管内皮细胞 VEGF 和 SCF 分泌的影响
- Author:
Shan HE
;
Liyue ZHAO
;
Xiaoye BA
;
Baoyan WANG
;
Jianling SONG
- Publication Type:Journal Article
- Keywords:
rat;
bone marrow mesenchymal stem cells (rBMSCs);
vascular endothelial cells (rVECs);
indirect co-culture;
angiogenesis;
phenytoin (PHT)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2016;(2):204-208,225
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:Objective To investigate the effects of phenytoin (PHT)on the secretion of vascular endothelial growth factor (VEGF)and stem cell factor (SCF)based on the establishment of indirect co-culture system of rat bone marrow mesenchymal stem cells (BMSCs)and vascular endothelial cells (VECs).Methods Indirect co-culture model of rat BMSCs and VECs was established.Experimental groups:indirect co-culture groups (PHT concentrations were 0,20 and 40 μg/mL);the control group:BMSCs culture group and VECs culture group (PHT concentrations were 0,20 and 40μg/mL).The contents of VEGF and SCF in the culture supernatant were measured using double antibody sandwich ABC-ELISA method on cultivation days 2,4,6.Results ELISA assay of the rBMSCs and rVECs in indirect co-culture supernatants,collected on culture days 2,4 and 6 showed that:① VEGF:On culture day 2,VEGF level in the co-culture groups was significantly higher than those in BMSCs group (P <0.05)and rVECs group (P <0.001).As culture time prolonged and PHT concentration increased to 20 μg/mL and 40 μg/mL,VEGF level increased too (P <0.001,P <0.05 ).② SCF testing results showed that the secretion of SCF in co-culture groups was higher than that in the control groups.When PHT was 20 μg/mL,the secretion of
SCF increased as the incubation time increased;but as the incubation time increased, PHT concentration of 40 μg/mL made SCF content decrease.Each group did not significantly differ (P > 0.05 ).Conclusion PHT promotes the secretion of VEGF and may reduce the secretion of SCF.
