Evaluation of Diagnostic Value of Antibody Against Aspergillus Fumigatus Pectate Lyase A for Invasive Aspergillosis
10.3969/j.issn.1671-7414.2016.01.002
- VernacularTitle:抗烟曲霉果胶裂解酶 A 抗体检测对侵袭性曲霉病诊断价值的评估
- Author:
Xiujuan SHANG
;
Guoyong ZHANG
;
Qian LIU
;
Xiao CHEN
;
Fangqiu LI
- Publication Type:Journal Article
- Keywords:
aspergillus fumigatus;
pectate lyase A;
IgG;
ELISA
- From:
Journal of Modern Laboratory Medicine
2016;(1):5-7,11
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an ELISA for detecting antibody against Aspergillus fumigatus pectate lyase A(anti-PlyA),and evaluate its diagnostic value for invasive aspergillosis (IA).Methods An indirect ELISA for IgG antibody a-gainst PlyA was established using PlyA as coating antigen.The serum from 97 IA patients,80 non-IA patients and 200 healthy donors were tested,the results were compared with anti-DPPV (antibody against Aspergillus fumigatus dipeptidyl peptidase V fragment)and anti-TR (antibody against Aspergillus fumigatus thioredoxin reductase).Results The intra-as-say coefficients of variation of the ELISA method for detecting anti-PlyA was 5.3%,and inter-assay coefficients of variation was 10.9%.The sensitivity and specificity of diagnosis of IA were 62.9% and 90.4%,respectively.The positive rates of an-ti-PlyA in non-neutropenic and neutropenic IA patients were 43.8% and 72.3%,respectively (χ2 =7.493,P <0.05).There was no significant difference between the positive rates of anti-PlyA (62.9%),anti-TR (60.8%),and anti-DPPV (67.0%) (χ2 =0.562,P > 0.05).When combined anti-PlyA,anti-TR,and anti-DPPV,the diagnostic sensitivity for IA patients in-creased to 92.8%.Conclusion An ELISA for detecting anti-PlyA was successfully established.The diagnostic value of these three kinds of antibody was superior in non-neutropenic IA patientsto that in neutropenic IA patients.The combined detec-tion of three antibodies could provide higher sensitivity.
